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S. Sandhya , J. Chandrasekhar , Banji David , K. R. Vinod

Abstract

Abrus precatorius Linn. popularly known as Crab's eye is a slender, woody perennial climber reported to have antioxidant, antibacterial, cytotoxic, anti-diabetic, anti-tubercular and anti-plasmodial activities. The present investigation was carried out to evaluate the hair growth promoting potentiality of aqueous extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of aqueous extract of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The aqueous extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300mg/kg which was comparable to that of 2%minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. A remarkable change in the mineral concentration in blood was not observed for treated group of animals. From the present investigation it was confirmed that Abrus precatorius is potent hair growth promoter and can be suggested to be an effective alternative to synthetic hair growth promoters.

Keywords

Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidil

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P. K. Gadhia ¹, Mohini Gadhia ², Shaji Georje ¹, K. R. Vinod ¹, Meonis Pithawala ³

Affiliations
1 Department of Biosciences, Veer Narmad South Gujarat University, Surat 395 007, IN
2 Department of Aquatic Biology, Veer Narmad South Gujarat University, Surat 395 007, IN
3 C. G. Bhakta Institute of Biotechnology, Gopal Vidyanagar, Bardoli 394 350

Abstract

The mitotic chromosomes from the gills of the fish Boleophthalmus dussumieri were studied for the induction of chromosomal aberrations after in vivo treatments with three separate antineoplastic antibiotics: Bleomycin (radiomimetic drug), Mitomycin-C (bifunctional alkylating agent) and Doxorubicin (intercalating agent). Fishes were directly exposed to these agents via intramuscular injections. Two separate treatment timings, 4 hours and 24 hours were selected for all antineoplastic treatments. The concentration doses of Bleomycin (150µg and 300µg), Mitomycin-C (1 µg, 2 µg and 4 µg) and Doxorubicin (10 µg, 20 µg and 40 µg) were decided on the basis of the body weight of fishes and earlier laboratory findings. The results revealed dose and time dependent increase in the chromosomal aberrations after treatments with all antineoplastic drugs. The type of aberrations observed include chromatid breaks, acentric fragments, dicentric and ring configurations. The findings add to the fact that the fish Boleophthalmus dussumieri is a suitable cytogenetic model for in vivo detection of potential mutagens.

Keywords

Boleophthalmus dussumieri, Chromosomes, Bleomycin, Mitomycin-c, Doxorubicin, Clastogenic Effects

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S. Sandhya ¹, S. A. H. Jafferi ², K. R. Vinod ², David Banji ², D. Narender Prasad ²

Affiliations
1 Dept. of Pharmacognosy, Nalanda College of Pharmacy, Nalgonda AP, IN
2 Nalanda College of Pharmacy, Nalgonda, A.P., IN

Abstract

The present study was undertaken to evaluate anthelmintic and larvicidal activity of crude ethanolic leaf extract of Zyziphus mauritiana belonging to family Rhamnaceae. Through the preliminary chemical tests it was found that the plant is rich in flavonoids, phenols, steroids and tannins. Pheretima posthuma was used as the test worms. Various concentrations of ethanolic extracts were tested in the anthelmintic screening, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate was included as standard reference and distilled water as control. In the case of larvicidal activity the study was conducted on Culex quniquefasicatus species of mosquito larvae and the rate of larval mortality was calculated. The results indicated that the crude ethanolic extract significantly demonstrated paralysis and also caused death of the helminth especially at higher concentration of 50 mg/ml, as compared to standard reference piperazine citrate. Similarly very optimistic results were observed for Culex quniquefasicatus species of mosquito larvae and LC50 value was calculated as 54.9658.

Keywords

Antihelmintic, Larvicidal, Zyziphus mauritiana, Pheretima Posthuma, Culex quniquefasicatus.

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K. R. Vinod ¹, S. Sandhya ²

Affiliations
1 Dept. of Pharmaceutics, Nalanda College of Pharmacy, Nalgonda– 508001, Anshra Pradesh, IN
2 Nalanda College of Pharmacy, Nalgonda-508001, Anshra Pradesh, IN

Abstract

The conventional experimental designs are not adequately contributing robustness of the experiment. Quality by design is an essential tool for time and cost effectiveness. Common design is factorial design. Factorial designing is more flexible and gives more knowledge about process and product and several software are also available. This manuscript gives the reader an in depth information of factorial experimental design, its theoretical background and how to implement this statistical tool in the real practical research.

Keywords

Total Quality Management, Quality by Design, Interaction, Factorial Design.

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K. R. Vinod ¹

Affiliations
1 Dept. of Pharmaceutics, Nalanda College of Pharmacy, Nalgonda 508001, A.P., IN

Abstract

This article gives staff and students to get an overall idea about GLP which is included in pharma curriculum. Separate QC procedures must be identified for raw materials, API, finished products and packaging and labelling. Safety instructions which can be implemented in the lab also is furnished. In addition to this, the duties and responsibilities of the lab in charge, lab attender and lab assistant of Institutions is differentiated.

Keywords

GLP, ISO, Triple Role Concept.

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S. A. H. Jafferi ¹, S. Sandhya ², K. R. Vinod ¹, D. Narender Prasad ¹, K. Venkataramana ³

Affiliations
1 Nalanda College of Pharmacy, Nalgonda, A.P, IN
2 Department of Pharmacognosy, Nalanda College of Pharmacy, Cherlapally, Nalgonda-508001 Andhra Pradesh, IN
3 ASN Pharmacy College, Tenali, A.P, IN

Abstract

There has always been a closed association between plants and human beings through ancient time and till date. Physalis angulata is an annual herb indigenous to many parts of the tropics, including the Amazon. It can be found on most continents in the tropics, including Africa, Asia, and the Americas. It grows up to 1 m high, bears small, cream-colored flowers, and produces small, light yellowish-orange, edible fruit sometimes referred to as Cutleaf Groundcherry. It is a popular medicine used due to its anti inflammatory effects, anti tumor effects, immunomodulatory effects etc. The present study is to evaluate the anti bacterial and anti platelet activity of the ethanolic leaf extract of Physalis angulata. From the phytochemical screening it was found that the plant is mainly constituted with phenols, steroids and flavonoids .The anti bacterial activity was performed by well diffusion method on various concentrations of the extract. Gentamycin was used as the standard and ethanol as the control. In the antiplatelet activity study, the platelet was induced using adenosine di phosphate. Nephlometer was used to observe the aggregation. It was observed that the ethanolic leaf extract showed pronounced anti platelet activity. But the anti bacterial activity was found to be false positive.

Keywords

Physalis angulata, Anti-Platelet, Anti Bacterial, Nephlometer.

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